Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.6K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
Riboswitches01:56

Riboswitches

8.4K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.4K
The Unfolded Protein Response01:37

The Unfolded Protein Response

5.0K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

990
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
990
Regulated Protein Degradation02:58

Regulated Protein Degradation

7.5K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential impact of proton pump inhibitors and antibiotics on immunotherapy efficacy after chemoradiotherapy in locally advanced non-small-cell lung cancer: a post-hoc analysis of the PACIFIC trial.

The Lancet. Oncology·2026
Same author

Spatial Biomarker Deep Learning Model Predicts Response to PI3K Inhibition in Head and Neck Cancer.

Cancers·2026
Same author

Genome-wide association study of never-smoking non-drinking young adults developing oral squamous cell carcinoma.

BMC cancer·2026
Same author

Going the distance: a cross-sectional geospatial analysis quantifying province-wide inequities in travel-based access, and fragility of access to French-language primary care provided by family physicians in Ontario, Canada.

BMJ open·2026
Same author

Adhesion G protein-coupled receptors.

Pharmacological reviews·2026
Same author

Profiling circular RNAs in amniotic fluid and fetal lungs from congenital diaphragmatic hernia cases: insights into potential prognostic and diagnostic applications.

Pediatric surgery international·2026
Same journal

Modeling and analysis of forward and inverse kinematics for a flexible Stewart platform.

PloS one·2026
Same journal

Barriers and facilitators to healthcare utilization amongst people living with sickle cell disease in the United States: A scoping review.

PloS one·2026
Same journal

Enhancing data completeness in time series: Imputation strategies for missing data using significant periodically correlated components.

PloS one·2026
Same journal

Key targets and mechanisms by which gut microbiota-derived metabolites regulate Alzheimer's disease through the immune - inflammatory pathway: Based on network pharmacology and molecular docking.

PloS one·2026
Same journal

Grid-tied Transformer-less Boost Switched Capacitor Topology (TLBSCT) for PV applications.

PloS one·2026
Same journal

The load-velocity profiles and exercise-specific velocity zones for seven commonly used weightlifting exercises.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Aug 30, 2025

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

8.4K

Functionally impaired isoforms regulate TMPRSS6 proteolytic activity.

Sébastien P Dion1,2, Antoine Désilets1,2, Gabriel Lemieux1,2

  • 1Department of Pharmacology-Physiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Plos One
|August 31, 2022
PubMed
Summary
This summary is machine-generated.

The transmembrane serine protease TMPRSS6 has four isoforms. Isoforms 3 and 4 inhibit isoform 2 activity, impacting iron homeostasis and TfR1 shedding.

More Related Videos

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

4.2K
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

2.6K

Related Experiment Videos

Last Updated: Aug 30, 2025

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

8.4K
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

4.2K
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

2.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Iron Metabolism

Background:

  • TMPRSS6 (transmembrane serine protease 6) is crucial for iron homeostasis.
  • It exists in four human isoforms, with isoform 2 known to regulate hepcidin production.
  • The roles of the catalytically impaired isoforms 3 and 4 remain largely uncharacterized.

Purpose of the Study:

  • To investigate the functions of TMPRSS6 isoforms 3 and 4.
  • To explore the interactions between different TMPRSS6 isoforms.
  • To identify novel protein partners of TMPRSS6 and their roles in iron regulation.

Main Methods:

  • Co-expression of TMPRSS6 isoforms and target proteins (e.g., TfR1) in cellular systems.
  • Analysis of proteolytic activity and protein cleavage.
  • Identification of protein-protein interactions using biochemical assays.

Main Results:

  • TMPRSS6 isoforms 3 and 4 were found to inhibit the proteolytic activity of TMPRSS6 isoform 2.
  • These isoforms can interact with each other.
  • Forty-nine potential protein partners for TMPRSS6 isoforms were identified, including transferrin receptor 1 (TfR1).
  • Co-expression studies demonstrated that TfR1 is cleaved and shed from the cell surface by TMPRSS6.
  • Isoforms 3 and 4 exhibited dominant-negative effects.

Conclusions:

  • TMPRSS6 isoforms 3 and 4 modulate the activity of isoform 2, suggesting a complex regulatory mechanism.
  • TMPRSS6 interacts with and cleaves TfR1, providing new insights into iron regulation pathways.
  • The dominant-negative behavior of isoforms 3 and 4 highlights their potential roles in cellular signaling and disease.