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Identification of Lsd1-interacting non-coding RNAs as regulators of fly oogenesis

Tzu-Ling Shao1, Ruei-Teng Ting1, Ming-Chia Lee1

  • 1Department of Life Sciences and Institute of Genome Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.

Cell Reports
|August 31, 2022
PubMed

Insights

Long noncoding RNAs (lncRNAs) interact with Lysine-specific demethylase 1 (LSD1) to regulate cell differentiation. This study identifies ovarian lncRNAs (LINRs) that are crucial for oogenesis and germline stem cell differentiation in vivo.

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Molecular Biology

Background:

  • Lysine-specific demethylase 1 (LSD1) is crucial for cell proliferation and differentiation.
  • LSD1 interacts with long noncoding RNAs (lncRNAs) in cancer, but its role in cell-specific differentiation in vivo is unclear.

Purpose of the Study:

  • To investigate the in vivo role of lncRNA-LSD1 interactions in cell differentiation.
  • To identify novel lncRNAs interacting with LSD1 in ovarian tissues.

Main Methods:

  • RNA immunoprecipitation sequencing (RIP-seq) to identify LSD1-interacting RNAs.
  • Genetic knockout of identified lncRNAs in Drosophila melanogaster.
  • Analysis of oogenesis and stem cell differentiation in mutant flies.

Main Results:

  • Three LSD1-interacting non-coding RNAs (LINRs) were identified in fly ovaries.
  • Knockout of LINR-1 and LINR-2 impaired fly egg production and hatch rate.
  • LINR-2 regulates germline stem cell and progenitor differentiation by modulating LSD1.

Conclusions:

  • Ovarian LINRs are essential for oogenesis and stem cell differentiation.
  • This study reveals a physiological role for lncRNA-LSD1 interplay in regulating differentiation.
  • Identified LINRs provide a model for studying dynamic lncRNA-LSD1 interactions in vivo.
Keywords:
CP: Developmental biologyCP: Molecular biologyLsd1Lsd1-interacting noncoding RNAfly oogenesisfollicle progenitorsgermline stem cellslong hairpin RNAslysine-specific demethylase 1

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