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Related Experiment Video

Updated: Aug 30, 2025

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

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Improving gene expression analysis efficacy from formalin-fixed paraffin embedded tissues.

Pantelis Dimaras1, Oskan Tasinov1, Desislava Ivanova1

  • 1Prof. Dr P. Stoyanov Medical University, Varna, Bulgaria.

Folia Medica
|August 31, 2022
PubMed
Summary
This summary is machine-generated.

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Optimizing RNA extraction and cDNA synthesis from FFPE tissues enhances gene expression analysis. Combining lysis buffer with phenol-based extraction and cDNA preamplification improves yield, sensitivity, and reproducibility for accurate diagnostics.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Advancements in understanding disease molecular basis necessitate improved RNA isolation and cDNA synthesis.
  • Higher quantity and quality of genetic material are crucial for diagnostic tests and experiments.

Purpose of the Study:

  • To compare three modified RNA extraction methods from formalin-fixed paraffin-embedded (FFPE) tissues.
  • To evaluate different cDNA synthesis strategies for gene expression studies.

Main Methods:

  • RNA extraction using lysis buffer, phenol-based extraction, or a combination with silica spin columns.
  • cDNA synthesis with oligo dT, oligo dT/random hexamer, or gene-specific primers.
  • RT-qPCR analysis on preamplified and non-preamplified cDNA templates.
Keywords:
FFPERNA probebiopsycDNAqPCR

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Related Experiment Videos

Last Updated: Aug 30, 2025

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

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Main Results:

  • Combined lysis buffer and phenol-based extraction yielded higher RNA.
  • cDNA preamplification increased qPCR detection confidence, efficiency, and sensitivity.
  • Improved RNA yield and cDNA synthesis enhanced quantitative gene expression analysis.

Conclusions:

  • The combined approaches significantly improved reproducibility and validity of quantitative gene expression analyses from FFPE tissues.
  • Optimized RNA extraction and cDNA synthesis are vital for reliable molecular diagnostics.