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Updated: Aug 30, 2025
Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
Published on: September 14, 2018
Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
Tomohiro Fujii1, Calliste Reiling2, Colette Quinn3
1Ajinomoto Bio-Pharma Services, 11040 Roselle Street, San Diego, CA 92121, United States.
Aim:
Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted.
Methods:
Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison.
Results:
Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation.
Conclusions:
The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies.
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