Composition and Characterization of Cold Pressed Moringa oleifera Seed Oil

The present study aims to investigate the volatile compound and the triacylglycerol profiles of Tunisian cold pressed Moringa oleifera seed oil (MoSO) and to assess its thermal properties and its biological activities. GC-MS analysis identified thirty six phyto-compounds amounting to 98.99% of the total oil. These compounds were classified into eleven groups among which the fatty acid one exhibited the highest intensity (91.63%). Cis, 6-octadecenoic acid was the most abundant compound (70.68%). The triacylglycerol composition of MoSO was characterized by the predominance of the glycerol trioleate (OOO) (32.42±0.12%). Thermogravimetric analysis of MoSO showed that the oil possess an interesting thermal stability with a highly Onset temperatures (T_onset) of 390.72°C and 357.47°C, respectively in nitrogen and air atmospheres. By using the ABTS assay, MoSO exhibited an interesting antioxidant capacity of 365 μM TEAC. The oil was also endowed with a relatively strong anti-inflammatory activity since its treatment at the different concentrations tested (75, 150 and 300 μg/mL). However, no antimicrobial activity was observed. On the basis of the obtained results, MoSO could be used in diverse industrial applications such as pharmaceutical, cosmetic, and food fields thanks to its thermal stability and interesting biological activities.