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Comparability of Steroid Collision Cross Sections Using Three Different IM-HRMS Technologies: An Interplatform Study.

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This study compared collision cross section (CCS) measurements for steroids across three major ion mobility-mass spectrometry (IM-MS) platforms. Results show high interlaboratory reproducibility, crucial for steroid identification.

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Area of Science:

  • Analytical Chemistry
  • Spectrometry
  • Biochemistry

Background:

  • Steroids are vital biomolecules with numerous isomers, posing identification challenges.
  • Ion mobility-mass spectrometry (IM-MS) offers collision cross section (CCS) for enhanced steroid identification.
  • Existing CCS databases lack comprehensive comparisons across different IM-MS technologies.

Purpose of the Study:

  • To conduct a systematic interlaboratory comparison of CCS values from major commercial IM-MS platforms.
  • To evaluate the reproducibility and biases of CCS measurements across drift tube (DTIM-MS), traveling wave (TWIM-MS), and trapped ion mobility (TIM-MS) instruments.
  • To establish instrument-specific CCS databases for a set of 87 steroids.

Main Methods:

  • An interlaboratory study involving 142 CCS values for 87 steroids.
  • Measurements were performed using drift tube (DTIM-MS), traveling wave (TWIM-MS), and trapped ion mobility (TIM-MS) platforms.
  • Statistical analysis of CCS biases and reproducibility between instruments.

Main Results:

  • Excellent interlaboratory performance observed for 95% of ions, with CCS biases within ±1% (TIM-MS) and ±2% (TWIM-MS) relative to DTIM-MS.
  • A small fraction of ions (<1.5%) exhibited larger biases (up to 7%), highlighting the need to investigate ion conformation differences.
  • Three instrument-specific CCS databases were generated.

Conclusions:

  • IM-MS provides highly reproducible CCS values for steroid analysis, suitable for unambiguous identification.
  • Minor systematic differences exist between CCS values from different IM-MS analyzers, impacting non-targeted analysis.
  • This study represents the most comprehensive comparison of CCS from three IM-MS technologies for steroids and small molecules.