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Related Experiment Video

Updated: Aug 30, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Development of a paper-based lateral flow prothrombin assay.

Jerro Saidykhan¹, Louise Pointon², Stefano Cinti³

¹Department of Applied Sciences, Centre for Research in Biosciences (CRIB), University of the West of England, Coldharbour Lane, Bristol, BS16 1QY, UK. tony.killard@uwe.ac.uk.

Analytical Methods : Advancing Methods and Applications

|September 1, 2022

Summary

A new, low-cost paper-based assay accurately measures prothrombin time (PT) for diagnosing bleeding and clotting disorders. This simple diagnostic tool is ideal for resource-limited settings, improving patient care globally.

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

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Published on: March 9, 2017

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Last Updated: Aug 30, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Area of Science:

Biomedical Engineering
Point-of-Care Diagnostics
Hemostasis Assays

Background:

Disorders of hemostasis cause significant global morbidity and mortality.
Conventional prothrombin time (PT) tests are unsuitable for low-resource settings.
Paper-based diagnostics offer a promising solution for accessible healthcare.

Purpose of the Study:

To develop a simple, low-cost, paper-based lateral flow assay for prothrombin time (PT) measurement.
To optimize the assay for reliable performance in resource-limited environments.
To evaluate the assay's correlation with standard laboratory PT methods.

Main Methods:

Utilized wax printing on chromatography paper to create lateral flow channels.
Deposited thromboplastin reagent and calcium chloride onto paper strips.
Measured plasma sample flow rates to determine PT, optimizing paper type, reagent concentration, and sample volume.

Main Results:

The optimized paper-based assay demonstrated a 6 mm dynamic range for normal and factor-deficient plasmas.
Showed good correlation with a laboratory PT assay (Yumizen G200) in artificial plasmas (r² = 0.8112).
Exhibited good dynamic range and correlation with hospital PT in patient samples (r² = 0.7209).

Conclusions:

Developed a cost-effective, paper-based lateral flow assay for PT measurement.
The assay is suitable for use in low-resource settings, offering improved accessibility.
This technology has the potential to enhance the diagnosis and management of hemostasis disorders worldwide.