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Related Experiment Video

Updated: Aug 30, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon.

Haibi Wang¹, Amy Smith¹, Amelia Lovelace²

¹Department of Plant Pathology, University of Georgia, Athens, Georgia, United States of America.

|September 1, 2022

Summary

Pattern Triggered Immunity (PTI) in Arabidopsis thaliana affects Pseudomonas syringae pv. tomato DC3000 (Pto) gene expression. AlgU, a sigma factor, regulates bacterial stress response and virulence genes during plant infection.

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Area of Science:

Microbiology
Plant Pathology
Genomics

Background:

Pattern Triggered Immunity (PTI) is a plant defense mechanism.
The sigma factor AlgU regulates a large number of genes in Pseudomonas syringae pv. tomato DC3000 (Pto).

Purpose of the Study:

To identify the AlgU regulon within the plant host.
To determine the overlap between AlgU-regulated and PTI-regulated genes in Pto.

Main Methods:

RNA-sequencing was used to analyze transcriptomic data.
Comparative analysis of gene expression under PTI and mock conditions.

Main Results:

AlgU regulates 224 upregulated and 154 downregulated genes in Pto during Arabidopsis infection.
AlgU controls stress response and virulence genes, while downregulating flagellar motility genes.
PTI significantly alters the expression of over half of AlgU-regulated genes.

Conclusions:

AlgU plays a crucial role in modulating bacterial gene expression during plant infection.
PTI impacts the AlgU regulon, affecting bacterial adaptation and virulence within the host.