Intracellular replication of Inquilinus limosus in bronchial epithelial cells

Related Experiment Video

Updated: Nov 7, 2026

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
13:54

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

10.6K

Yanina Andrea Lamberti1, Martina Debandi1, Mariela Del Carmen Carrica1

  • 1CINDEFI (UNLP CONICET La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.

Microbial Pathogenesis
|September 1, 2022
PubMed

Insights

Inquilinus limosus, a resilient pathogen, invades and replicates within human bronchial epithelial cells. This intracellular survival mechanism may contribute to antibiotic resistance and chronic infections in cystic fibrosis patients.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Inquilinus limosus is an emerging multi-resistant opportunistic pathogen.
  • It primarily affects cystic fibrosis patients, leading to respiratory issues.

Purpose of the Study:

  • To investigate the interaction between Inquilinus limosus and human bronchial epithelial cells (16HBE14o-).
  • To understand the intracellular survival mechanisms of I. limosus.

Main Methods:

  • Confocal microscopy was used to study bacterial internalization.
  • Antibiotic protection assays were performed to assess bacterial survival and replication.
  • Cytokine production (IL-6, IL-8) was measured to evaluate host immune response.

Main Results:

  • Nearly 100% of attached I. limosus were internalized into acidic LAMP2-positive compartments.
  • I. limosus survived and replicated within these intracellular compartments.
  • The pathogen did not induce IL-6 or IL-8 production, suggesting immune evasion.

Conclusions:

  • Inquilinus limosus exhibits pathogenic properties by surviving intracellularly within epithelial cells.
  • This intracellular survival can lead to antibiotic treatment failure and chronic infections.
  • Understanding these mechanisms is crucial for developing new therapeutic strategies.
Keywords:
Cystic fibrosisInquilinus limosusIntracellular survivalPersistence

More Related Videos

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells09:12

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells

Published on: July 22, 2016

19.5K
Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium07:40

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Published on: February 21, 2025

630

Related Concept Videos

Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...