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Related Experiment Video

Updated: Aug 30, 2025

Fluorescence Imaging with One-nanometer Accuracy FIONA

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Published on: September 26, 2014

DNA-PAINT MINFLUX nanoscopy.

Lynn M Ostersehlt¹, Daniel C Jans^1,2, Anna Wittek^1,2

¹Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

|September 1, 2022

Summary

This summary is machine-generated.

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy now supports multiple colors by combining with DNA-PAINT labeling. This breakthrough enables advanced imaging of multiple molecular targets in biological samples.

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Area of Science:

Biophysics
Molecular Imaging
Cell Biology

Background:

MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy achieves high-resolution 3D imaging.
Current MINFLUX is limited to two colors due to its on-off switching mechanism.

Purpose of the Study:

To expand MINFLUX nanoscopy to image multiple molecular targets simultaneously.
To demonstrate a novel combination of MINFLUX with DNA-PAINT.

Main Methods:

Sequential multiplexing using DNA-PAINT was integrated with MINFLUX.
The combined technique was used for multicolor imaging.

Main Results:

MINFLUX was successfully combined with DNA-PAINT for multicolor super-resolution microscopy.
Three-color recordings of mitochondria in human cells were achieved.

Conclusions:

The integration of DNA-PAINT with MINFLUX overcomes previous color limitations.
This advancement enables multicolor super-resolution imaging of complex biological systems.