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Updated: Aug 30, 2025
Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster
Published on: December 13, 2014
Protocol for quantitative ethology on natural social interactions in Drosophila
Jing Ning1, Zhou Li1, Xingjiang Zhang1
1College of Life Sciences, Zhejiang University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China.
Abstract:
Quantitative ethology of social interactions between free-moving animals enables precise measurement of behavioral kinematics critical for various disciplines such as neuroscience. Here, we describe a set of tools for quantitative ethology of social interactions including the analysis pipeline SoAL, the training dataset SDPD, and the camera control software MIAS, along with experimental details. These tools are directly applicable for courtship behavior in Drosophila and can be used for other social interactions in other species after modification. For complete details on the use and execution of this protocol, please refer to Ning et al. (2022).
