In Situ Assay of Interfacial Interaction between ZnO Nanoparticles and Live Cell Disturbed by Surfactants

The interfacial interaction between pollutants and organisms is a critical process in controlling the environmental fates of pollutants; however, in situ assay of the interaction is still a great challenge. Here, in situ determination of dissociation constants (K_d) for ZnO nanoparticles (ZnO NPs) from live algal cells disturbed by different-charged surfactants was established using microscale thermophoresis (MST). Moreover, in situ measurement of the adhesion force between the ZnO NPs probe and live single cell was performed using an atomic force microscope (AFM). Results showed that the cationic cetyltrimethylammonium chloride (CTAC) and anionic sodium dodecylbenzenesulfonate (SDBS) increased but nonionic Triton X-100 (TX-100) decreased the adhesion of ZnO NPs on cells. However, the force signature exhibited a smooth single retracted peak at short distances in the SDBS- and TX-100-treated groups, distinguished from the "see-saw" pattern peak in the CTAC-treated groups. The extended Derjaguin-Landau-Verway-Overbeek (XDLVO) calculation further confirmed that SDBS and TX-100 mainly disturbed the short-range hydration on the NP-cell interface, while CTAC reduced the long-range electrostatic repulsion. Furthermore, an excellent linear correlation between Zn bioaccumulation and two parameters (K_d and adhesion force) indicated that NP-cell interfacial interactions affected Zn bioaccumulation. Thus, in situ assay provides a quantitative basis for the pollutant-organism interfacial interaction to evaluate the environmental fate and ecological risk of pollutants.