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Integral-mass balance method for determination of solvent drag reflection coefficient.

M B Wolf, P D Watson, D R Scott

    The American Journal of Physiology
    |July 1, 1987
    PubMed
    Summary

    The integral-mass balance (IMB) method accurately measures the solvent drag reflection coefficient (sigma f) for macromolecular transport. This method corrects for errors, ensuring reliable data for microvascular studies.

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    Area of Science:

    • Physiology
    • Biophysics
    • Biomedical Engineering

    Background:

    • Transcapillary macromolecular transport is crucial for organ function.
    • Accurate measurement of the solvent drag reflection coefficient (sigma f) is essential for understanding this process.
    • Existing methods may be limited in accuracy and scope.

    Purpose of the Study:

    • To introduce and validate the integral-mass balance (IMB) method for measuring sigma f.
    • To assess the impact of theoretical and experimental factors on IMB method accuracy.
    • To determine sigma f for albumin and plasma proteins in skeletal muscle.

    Main Methods:

    • Developed the integral-mass balance (IMB) method using cumulative water and macromolecule movement.
    • Analyzed effects of low Peclet number, measurement errors, and systematic errors (leakage, hemolysis, evaporation, osmolality).

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  • Validated the IMB method in an isolated, perfused cat hindlimb preparation.

    • Main Results:

    • The IMB method accurately quantifies sigma f for transcapillary macromolecular transport.
    • Identified and quantified overestimations of sigma f caused by various error sources.
    • Demonstrated that these errors can be corrected and minimized through experimental design.
    • Obtained mean sigma f values of 0.82 +/- 0.08 for albumin and 0.83 +/- 0.02 for plasma proteins.

    Conclusions:

    • The integral-mass balance (IMB) method provides a valid and accurate approach to measure the solvent drag reflection coefficient (sigma f).
    • The method is robust against common experimental errors, which can be corrected.
    • Results support the IMB method's utility in studying macromolecular transport in various organs.