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Related Experiment Video

Updated: Aug 30, 2025

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Colorimetric Cotton Swab for Viral Protease Detection.

Ranit Dutta, Sparsh Makhaik, Peiyao Zhao

    Analytical Chemistry
    |September 2, 2022
    PubMed
    Summary

    A new colorimetric method detects active SARS-CoV-2 infection by reporting viral protease activity. This simple test uses a cotton swab for rapid, visual detection, aiding in distinguishing active from resolved infections.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Virology

    Background:

    • Accurate detection of active viral infections is crucial for effective treatment and public health strategies.
    • Distinguishing between active and resolved infections requires sensitive diagnostic tools that report viral activity.

    Purpose of the Study:

    • To develop a simple, rapid, point-of-care colorimetric assay for detecting the activity of a specific viral protease.
    • To establish a proof-of-concept using the SARS-CoV-2 main protease (Mpro) for active infection detection.

    Main Methods:

    • Design and synthesis of a novel chromophore reporter (CoV2-AMG) based on 4-aminomalachite green (4-AMG).
    • Demonstration of selective color conversion upon interaction with SARS-CoV-2 Mpro.
    • Evaluation of visual and smartphone-based detection capabilities.

    Main Results:

    • The CoV2-AMG reporter exhibited high selectivity for SARS-CoV-2 Mpro, with no observable color change for other viral proteases.
    • A distinct color change was observed, detectable by the naked eye and smartphone imaging.
    • The assay demonstrated a low limit of detection, indicating high sensitivity.

    Conclusions:

    • A straightforward colorimetric method for reporting viral protease activity has been developed.
    • This approach offers a promising strategy for rapid point-of-care detection of active SARS-CoV-2 infections.
    • The method's simplicity and generalizability hold potential for combating future viral outbreaks.