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Effect of intrauterine ozone therapy on Asherman syndrome, an experimental rat model
Seval Yılmaz Ergani1, Berna Dilbaz1, Hasan Murat Ergani2
1Department of Reproductive Endocrinology and Infertility, University of Health Sciences, Etlik Zubeyde Hanım Women's Health Training and Research Hospital, Ankara, Turkey.
Background/Aims:
Evaluate the effect of ozone therapy alone or in combination with estradiol in management of Asherman syndrome in a rat model.
Materials And Methods:
48 rats were used and divided into eight groups. In all the groups right uterine horn was used for the experiment while the left horns received no action. Groups A underwent two operations, and groups B three times each. Group 1A and 1B were determined as the sham group. Ozone gas was given to Group 2 alone; estradiol orally was given to Group 3 alone; estradiol and ozone gas together was given to Group 4.
Results:
In ozone therapy group; the diameter of the uterus, the uterine lumen and the uterine glands were increased, the endometrial epithelium was thicker in the group that received double ozone application, and the number and diameter of the endometrial gland increased in the combined use of ozone and estradiol. In the rats who underwent two surgeries, the maximum number of pregnancies and advanced pregnancies were observed in the left uterine horn. The minimum number of pregnancies were achieved in estradiol alone group. In the group receiving combined treatment with ozone and estradiol, pregnancies were observed at a higher gestational age.
Conclusion:
Ozone had a positive effect on the treatment of Asherman syndrome and has a significant impact on achieving pregnancy.
