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Published on: October 25, 2017
The effect of adjacent double-strand DNA on the G-triplex-ThT complex fluorescence intensity enhancement and its
Chengke Wang1, Jiangyu Li2, Ping Wang3
1Institute of Bionanotechnology, Ludong University, Yantai, 264025, PR China; College of Food Engineering, Ludong University, Yantai, 264025, PR China; College of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
Abstract:
In this paper, we have found that adjacent double-strand DNA (dsDNA) can enhance the fluorescence intensity of the G-triplex (G31)-thioflavin T (ThT) complex. By connecting the newly formed dsDNA with the G31 sequence, terminator nopaline synthase (TNOS) gene and Hg2+ were detected. The intermolecular duplex hybridization (e.g., TNOS gene and its complementary DNA), or the intramolecular mismatched thymine (T)-Hg2+-T pairs induced the formation of dsDNA with planar morphology, which resulted in the increased binding capacity of adjacent G31 to ThT, as well as the enhanced fluorescence intensity of G31-ThT complex. A versatile 'turn-on' fluorescence scaffold was developed for discriminating transgenic and non-transgenic soybeans and detecting Hg2+ in lake water.
