Developing a third-degree burn model of rats using the Delphi method

Area of Science:

• Biomedical research
• Animal models
• Burn treatment

Background:

• Animal experiments are crucial for burn research and treatment.
• Current methods for creating animal burn models lack standardization and scientific rigor.
• Existing models often have limited reference value, hindering research progress.

Purpose of the Study:

• To establish a standardized third-degree burn model in rats.
• To provide a scientifically rigorous and reliable reference model for burn research.
• To improve the quality and consistency of animal models in burn studies.

Main Methods:

• Utilized the Delphi method with expert consultations (n=13) over two rounds.
• Applied the boundary value method for indicator screening, modification, and supplementation.
• Determined indicator weights and established a comprehensive index system for the burn model.

Main Results:

• Achieved high expert consensus (100% and 88.67% positive coefficients).
• Expert authority coefficients were 0.73 and 0.67, with significant expert coordination (P<0.001).
• Established a detailed index system comprising 8 first-degree, 14 second-degree, and 46 third-degree indexes.

Conclusions:

• Successfully developed a standardized third-degree burn model in rats.
• The established model offers improved scientific rigor and reliability for burn research.
• This standardized model is expected to enhance overall proficiency in burn research quality and consistency.