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Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking.

Zhenfeng Yu1, Olena Vepris1, Christina Eich2

  • 1Translational Nanobiomaterials and Imaging Group, Department of Radiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, the Netherlands.

Mikrochimica Acta
|September 3, 2022
PubMed
Summary
This summary is machine-generated.

Upconversion nanoparticles (UCNPs) carrying antigens and adjuvants effectively stimulate dendritic cells (DCs) for immunotherapy. This platform enables simultaneous immune monitoring and tracking via upconversion luminescence imaging, demonstrating its potential for cancer treatment.

Keywords:
Dendritic cellsImmunotherapyIn vivo monitoringOVAPam3CSK4Upconversion nanoparticles

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Area of Science:

  • Nanotechnology
  • Immunology
  • Biomedical Imaging

Background:

  • Upconversion nanoparticles (UCNPs) convert near-infrared light to visible light, offering deep tissue penetration and low biological noise.
  • UCNPs' optical properties and surface modifiability make them suitable for targeted antigen delivery to dendritic cells (DCs) and molecular imaging.

Purpose of the Study:

  • To develop a novel nano-delivery platform using UCNPs for dendritic cell (DC)-based immunotherapy.
  • To enable simultaneous immune monitoring and molecular imaging of the therapeutic process.

Main Methods:

  • Chemically linking OVA 254-267 (OVA24) peptide antigen and Pam3CSK4 adjuvant to UCNPs via amide condensation.
  • Characterizing the synthesized OVA24-Pam3CSK4-UCNPs for morphology and surface properties.
  • Evaluating in vitro immune responses (DC maturation, T cell activation, IFN-γ production) and in vivo tracking using upconversion luminescence (UCL) imaging.

Main Results:

  • Synthesized UCNPs exhibited homogeneous morphology and good dispersion due to surface electronegativity.
  • In vitro studies confirmed that OVA24-Pam3CSK4-UCNPs effectively induced DC maturation, T cell activation, proliferation, and interferon gamma (IFN-γ) production.
  • In vivo UCL imaging successfully tracked UCNPs from peripheral sites to lymph nodes, demonstrating efficient delivery.

Conclusions:

  • The developed OVA24-Pam3CSK4-UCNPs serve as an effective nano-delivery platform for DC-based immunotherapy.
  • This platform facilitates simultaneous antigen delivery, immune stimulation, and real-time imaging for therapeutic monitoring.