Related Experiment Video
Updated: Aug 29, 2025

DNA-based Fish Species Identification Protocol
Published on: April 28, 2010
Rapid identification of fish species by laser-induced breakdown spectroscopy and Raman spectroscopy coupled with
Lihui Ren1, Ye Tian2, Xiaoying Yang2
1College of Physics and Optoelectronic Engineering, Ocean University of China, Qingdao 266100, China; Single-Cell Center, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Abstract:
There has been an increasing demand for the rapid verification of fish authenticity and the detection of adulteration. In this work, we combined LIBS and Raman spectroscopy for the fish species identification for the first time. Two machine learning methods of SVM and CNN are used to establish the classification models based on the LIBS and Raman data obtained from 13 types of fish species. Data fusion strategies including low-level, mid-level and high-level fusions are used for the combination of LIBS and Raman data. It shows that all these data fusion strategies offer a significant improvement in fish classification compared with the individual LIBS or Raman data, and the CNN model works more powerfully than the SVM model. The low-level fusion CNN model provides a best classification accuracy of 98.2%, while the mid-level fusion involved with feature selection improves the computing efficiency and gains the interpretability of CNN.
More Related Videos
Related Concept Videos
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

