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Related Concept Videos

Proteomics01:33

Proteomics

7.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: Aug 29, 2025

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Tissue-Characteristic Expression of Mouse Proteome.

Tian Lu1, Liujia Qian2, Yuting Xie2

  • 1School of Life Sciences, Fudan University, Shanghai, China; Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China; Westlake Intelligent Biomarker Discovery Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.

Molecular & Cellular Proteomics : MCP
|September 4, 2022
PubMed
Summary

Researchers created a comprehensive mouse proteome map covering 41 organs, identifying unique proteins and expression patterns. This resource aids biomedical research and understanding organ function and transplantation.

Keywords:
antioxidant enzymesdata independent acquisitionimmune privilegelateralitymousemulti-organproteomespectral librarytestistissue-specific proteins

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Area of Science:

  • Proteomics
  • Biomedical Research
  • Comparative Organ Analysis

Background:

  • Mice are crucial model organisms in biomedical research.
  • A comprehensive proteomic understanding of mouse organs is lacking.
  • Existing spectral libraries are incomplete.

Purpose of the Study:

  • To establish a comprehensive spectral library for mouse organs.
  • To generate quantitative proteome maps for 41 mouse organs using data-independent acquisition.
  • To identify novel proteins and characterize tissue-specific proteomic profiles.

Main Methods:

  • Utilized data-independent acquisition mass spectrometry.
  • Quantified proteomes across 41 distinct mouse organs.
  • Developed a comprehensive mouse spectral library.

Main Results:

  • Generated proteome maps for 41 mouse organs, including rare tissues.
  • The spectral library contains 178,304 peptides from 12,320 proteins, with 1678 novel entries.
  • Nervous and immune system organs showed distinct proteomes.
  • Identified characteristic protein expression in immune-privileged organs.
  • Discovered tissue-specific proteins, notably in the testis.
  • Observed left-right asymmetry in paired organs for antioxidant enzymes and functional proteins.

Conclusions:

  • The study provides a valuable, comprehensive spectral library and quantitative proteome resource for mouse studies.
  • Distinct proteomic profiles were identified across various mouse organs, offering insights into their functions.
  • Findings on immune-privileged organs and organ asymmetry may inform transplantation and developmental biology research.