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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Aug 29, 2025

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
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Robust Chemical Strategy for Stably Labeling Polyester-Based Nanoparticles with BODIPY Fluorophores.

Ivan S Alferiev1, Ilia Fishbein1, Robert J Levy1

  • 1Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104-4318, United States; The University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104-4318, United States.

ACS Applied Polymer Materials
|September 5, 2022
PubMed
Summary

Covalently attaching BODIPY fluorophores to biodegradable polyester nanoparticles creates traceable imaging agents. This method ensures stable labeling for monitoring nanoparticle fate in biomedical applications, improving drug delivery and diagnostics.

Keywords:
BODIPYconjugation chemistrycovalent labelingfluorescencenanoparticlepolylactic acid

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Nanotechnology

Background:

  • Aliphatic polyesters are widely used for biodegradable nanoparticles in biomedical applications.
  • Clinical translation necessitates reliable methods for tracking these nanoparticles in biological systems.

Purpose of the Study:

  • To develop a versatile strategy for covalently labeling poly(D,L-lactide) nanoparticles with BODIPY fluorophores.
  • To create traceable nanoparticles for monitoring their behavior in biological environments.

Main Methods:

  • Esterification of poly(D,L-lactide) terminal hydroxyl groups with carboxy-functionalized BODIPY dyes or amino acid linkers.
  • Conjugation of BODIPY dyes to amino acid-derivatized polymers after deprotection.
  • Comparison of covalently labeled versus encapsulated nanoparticles under various conditions.

Main Results:

  • Covalently labeled nanoparticles showed stable tracer association, unlike encapsulated dyes.
  • Internalization of covalently attached probes was irreversible, indicating active nanoparticle uptake.
  • The labeling strategy proved effective for creating traceable biodegradable nanoparticles.

Conclusions:

  • Conjugating BODIPY fluorophores to polymers provides an effective labeling strategy for biodegradable nanoparticles.
  • This approach facilitates the development of traceable nanoparticles for therapeutic and diagnostic applications.