Methylothon: a Versatile Course-Based High School Research Experience in Microbiology and Bioinformatics with Pink...

Area of Science:

• Microbial Ecology
• Molecular Biology
• Bioinformatics
• Science Education

Background:

• Methylothon is an inquiry-based learning module for high school students.
• It focuses on pink-pigmented, plant-associated methylotrophic bacteria.
• The module integrates microbial ecology, molecular biology, and bioinformatics.

Purpose of the Study:

• To present the learning goals and resources of the Methylothon module.
• To describe the adaptation of the module for remote learning during the COVID-19 pandemic.
• To share lessons learned for instructors and researchers interested in K-12 outreach.

Main Methods:

• The original in-person module involved isolating bacteria, PCR, sequencing, and phylogenetic analysis.
• The adapted version emphasized bioinformatics, made lab work optional, and utilized free web-based tools.
• Curriculum resources are publicly available at http://methylothon.com.

Main Results:

• The adapted module increased flexibility and accessibility.
• Student feedback indicated effectiveness in teaching BLAST and phylogenetic trees.
• Students valued hands-on work and community science participation.

Conclusions:

• The Methylothon module effectively introduces students to microbial ecology and bioinformatics.
• Adapting the curriculum for remote learning enhanced its reach and impact.
• The module serves as a valuable tool for science education and K-12 outreach.