Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of aptamer-triggered hybridization chain reaction for rapid visual ATP detection using gold nanoparticles.

Analytical methods : advancing methods and applications·2026
Same author

Design and Evaluation of Sticky End-Type Bivalent DNA Aptamers Containing M08s-1 as Anticoagulant Agents.

ChemMedChem·2025
Same author

Seasonal changes in daily temperature fluctuation control flowering through a time-dependent regulation of <i>FLOWERING LOCUS T</i> in <i>Arabidopsis</i>.

Research square·2025
Same author

Co-option and neofunctionalization of stomatal executors for defence against herbivores in Brassicales.

Nature plants·2025
Same author

LIPID RICH 1 modulates allocation of carbon between starch and triacylglycerol in Arabidopsis leaves.

Journal of experimental botany·2025
Same author

Crystallographic analysis of the Escherichia coli tRNA seleno-modification enzyme in complex with tRNA.

Acta crystallographica. Section F, Structural biology communications·2025

Related Experiment Video

Updated: Aug 29, 2025

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

14.0K

DNA-functionalized colloidal crystals for macromolecular encapsulation.

Maasa Yokomori1, Hayato Suzuki2, Akiyoshi Nakamura2

  • 1Center for Integrated Research of Future Electronics (CIRFE), Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. tagawa.miho@material.nagoya-u.ac.jp.

Soft Matter
|September 5, 2022
PubMed
Summary

Researchers developed DNA-functionalized gold nanoparticle crystals for encapsulating proteins. This breakthrough enables new possibilities for nanoscale drug delivery systems and advanced colloidal crystal engineering.

More Related Videos

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.4K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

10.9K

Related Experiment Videos

Last Updated: Aug 29, 2025

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

14.0K
Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.4K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

10.9K

Area of Science:

  • Nanotechnology and Materials Science
  • Biotechnology and Molecular Engineering

Background:

  • DNA-based structures offer potential for encapsulating nanoscale molecules like proteins for applications in reaction fields and drug delivery.
  • DNA-functionalized nanoparticle (DNA-NP) colloidal crystals are programmable DNA-based structures with metal nanoparticles and enhanced mechanical properties.
  • The encapsulation of guest molecules within DNA-NP crystal lattices is theoretically possible but lacks versatile strategies.

Purpose of the Study:

  • To develop DNA-functionalized gold nanoparticle (DNA-AuNP) crystals with tunable interparticle spacing for effective molecular encapsulation.
  • To demonstrate the feasibility of nanoscale encapsulation using DNA-NP crystals, specifically for protein delivery applications.

Main Methods:

  • Fabrication of DNA-AuNP crystals with controlled interparticle spacing.
  • Modification of DNA-AuNP crystals with functional moieties (biotin) to enhance molecular retention.
  • Characterization of crystal structure before and after encapsulation using small-angle X-ray scattering.
  • Encapsulation of CRISPR/Cas9 ribonucleoproteins by leveraging molecular affinity.

Main Results:

  • DNA-AuNP crystals with tunable spacing were successfully developed for molecular encapsulation.
  • Biotin modification effectively retained molecules within the DNA-AuNP crystals.
  • CRISPR/Cas9 ribonucleoproteins were successfully encapsulated into the DNA-AuNP crystals.
  • Small-angle X-ray scattering confirmed crystal integrity before and after molecular encapsulation.

Conclusions:

  • Metal-DNA hybrid crystals serve as viable carriers for direct protein delivery, demonstrated via biolistic bombardment.
  • This study presents a novel strategy for creating DNA-based structures for macromolecular encapsulation.
  • The findings offer a new research direction for colloidal crystal engineering utilizing DNA functionalities.