Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein Binding and Molecular Size Govern Molecular Transport into Dermal Interstitial Fluid.

ACS sensors·2026
Same author

Catheter-Electrode System for Continuous Aptamer-Based Sensing in the Rat Subcutaneous Space.

ECS sensors plus·2026
Same author

Multicompartment Drug Monitoring Reveals Paired Brain-Liver Kinetics and Selective Central Nervous System Barrier Permeability in Rats.

ACS sensors·2026
Same author

Interrogation of Small Molecules to Surface-Bound Aptamer Binding Kinetics with Electrochemical Aptamer-Based Sensors Using Intermittent Pulse Amperometry.

Analytical chemistry·2025
Same author

Investigation of effects of collection conditions on amino acid concentrations in sweat and correlations with their Circulating levels in plasma.

Scientific reports·2025
Same author

Extending the Operational Lifespan of Nucleic Acid-Based Electrochemical Sensors via Protection against Hydrogen Peroxide and Oligonucleotide Displacement.

ACS sensors·2025

Related Experiment Video

Updated: Aug 29, 2025

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.9K

Solution-Phase Electrochemical Aptamer-Based Sensors.

Yuchan Yuan, Ahilya Bali, Ryan J White

    IEEE Transactions on Bio-Medical Engineering
    |September 5, 2022
    PubMed
    Summary
    This summary is machine-generated.

    A novel solution-phase electrochemical aptamer-based sensor (EAB) approach improves longevity and sensitivity. This method, using freely mobile aptamers, shows promise for point-of-care diagnostics.

    More Related Videos

    Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
    13:15

    Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

    Published on: June 1, 2011

    33.7K
    A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
    12:31

    A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

    Published on: February 28, 2015

    15.3K

    Related Experiment Videos

    Last Updated: Aug 29, 2025

    Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
    09:33

    Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

    Published on: March 21, 2018

    9.9K
    Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
    13:15

    Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

    Published on: June 1, 2011

    33.7K
    A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
    12:31

    A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

    Published on: February 28, 2015

    15.3K

    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Biosensors

    Background:

    • Electrochemical aptamer-based sensors (EABs) show promise for in-vivo analyte detection.
    • Current EABs are limited by short longevity (24 hours) and challenges in detecting low analyte concentrations (nM range).

    Purpose of the Study:

    • To develop a novel solution-phase EAB sensor with improved longevity and sensitivity.
    • To adapt a molecular-beacon cortisol aptamer for an amperometric, solution-phase EAB sensor.

    Main Methods:

    • Utilized a redox-tagged aptamer (methylene-blue) for amperometric detection with interdigitated electrodes.
    • Developed a solution-phase EAB sensor where aptamers are freely mobile, not covalently bound to the electrode.
    • Employed a self-assembled monolayer alkylthiolate blocking layer on the gold working electrode.

    Main Results:

    • Achieved approximately 5% signal gain starting at 10 nM cortisol.
    • Demonstrated a saturated signal gain of approximately 70% at several μM cortisol.
    • The solution-phase approach showed basic feasibility but required a blocking layer, impacting longevity.

    Conclusions:

    • Solution-phase EAB sensors offer potential for improved longevity and sensitivity compared to surface-bound EABs.
    • This approach is promising for point-of-care testing applications.
    • Further development is needed for long-lasting continuous sensing applications.