Aminated β-Glucan with immunostimulating activities and collagen composite sponge for wound repair

Immunostimulating activities of yeast β(1 → 3)-D-Glucan (β-Glucan) mainly depended on its structures. However, due to the tight triple helix structure of β-Glucan, its immunostimulating activity is greatly weakened. Therefore, in order to partially unwind the tight triple helix structure of β-glucan and improve its solubility in the medium, we modified it by amination in this study (A-Glu). The results showed that A-Glu could stimulate Raw264.7 macrophages and significantly enhance its TNF-α, IL-6, and IL-10 cytokine expression levels in vitro. A-Glu could also induce a shift of M0 Raw264.7 toward M1, and M2 toward M1. To expand the application of A-Glu in wound repair, the composite sponge consisting of A-Glu and type I collagen via the formation of a stable polyion complex (PIC) was developed. Moreover, the composite sponge could accelerate wound repair significantly. These results reveal that soluble A-Glu as an immunostimulating agent has potential applications in biomedicine.