Related Experiment Video
Updated: Aug 29, 2025

Field-Based Thermal Physiology Assay: Cold Shock Recovery under Ambient Conditions
Published on: March 9, 2021
Effect of cold storage on some biological and physiological performance of Adalia decempunctata L
Zahra Mojib-Haghghadam1, Jalal Jalali Sendi2, Arash Zibaee2
1Department of Plant Protection, University Campus 2, University of Guilan, Rasht, Iran; Plant Protection Research Department, Guilan Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
Abstract:
Adalia decempunctata (L.) is a common predator in agro ecosystems. Here, the effects of low-temperature storage on biological parameters, energy reserves, and antioxidant enzymes were investigated. Eggs, larvae, and pupae of this predator were maintained for 1-4 weeks and adults for different days (20, 35, 50, and 65 d) at 4, 7, and 10 °C in darkness and 70 ± 5% R.H. After storage of 20 days, the survival rate in male and female insects was >90% in 4, 7, and 10 ± 1 °C. In 65 days, the highest survival rate for adult insects was recorded at 4 °C. Effect of cold storage on the performance of adult's A. decempunctata was not statistically different for periods up to 35 days, but decreased longer duration (65 days). The female adults were able to tolerate the cold storage period by producing higher amounts of glucose and antioxidant enzymes, thus displaying a high survival rate. Based on our results, maintaining A. decempunctata adults at low temperatures (i.e. 4, 7, and 10 °C) for 20 or 35 days performed best without loss of quality.
Related Concept Videos
Responses to Heat and Cold Stress
Decreased Body Temperature
Adaptations that Reduce Water Loss

