Related Experiment Video
Updated: Aug 29, 2025
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
Published on: June 7, 2018
Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by
Carmen M Anadon1, Chaomei Zhang2, Xuefeng Wang3
1Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33614, USA.
Abstract:
Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8+ TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).
