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Updated: Aug 19, 2026

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)
Published on: December 5, 2013
Macromolecular interaction and the electrophoretic mobility of esterase-5 from Drosophila pseudoobscura
Abstract:
Esterase-5 is one of the most polymorphic loci in Drosophila pseudoobscura. Some variants reportedly produce a dimeric enzyme, while a few produce a monomeric form. This paper reports the finding that during electrophoresis ESTERASE-5 exists in a dynamic equilibrium between monomers and dimers, an equilibrium that is dependent on the running temperature of the gels. This is shown by a series of analytical electrophoresis experiments in which the apparent molecular weights of several variants are determined at four different temperatures. Increasing temperatures result in a linear decrease in the logarithm of apparent molecular weights. Macromolecular interactions thus are a significant determinant of EST-5 electrophoretic mobility.
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