Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

521
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
521
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

518
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
518
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

1.5K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
1.5K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

1.2K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
1.2K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

2.7K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
2.7K
IR Spectrum Peak Broadening: Hydrogen Bonding01:23

IR Spectrum Peak Broadening: Hydrogen Bonding

1.1K
The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1.
However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially distributed complex organic matter detected in an ancient river valley in Jezero crater, Mars.

Science advances·2026
Same author

Simple Estimation of Errors and Multi-Pixel Signal-to-Noise Ratio for Gaussian, Lorentzian, and Pseudo-Voigt functions Fit to Noisy Spectral Data.

Applied spectroscopy·2026
Same author

Spectral Background Calibration of Scanning Habitable Environments with Raman and Luminescence for Organics and Chemicals (SHERLOC) Spectrometer Onboard the <i>Perseverance</i> Rover Enables Identification of a Ubiquitous Martian Spectral Component.

Applied spectroscopy·2024
Same author

Inorganic interpretation of luminescent materials encountered by the Perseverance rover on Mars.

Science advances·2024
Same author

Calibration of Raman Bandwidths on the Scanning Habitable Environments with Raman and Luminescence for Organics and Chemicals (SHERLOC) Deep Ultraviolet Raman and Fluorescence Instrument Aboard the <i>Perseverance</i> Rover.

Applied spectroscopy·2023
Same author

Aqueous alteration processes in Jezero crater, Mars-implications for organic geochemistry.

Science (New York, N.Y.)·2022

Related Experiment Video

Updated: Aug 29, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.2K

A Model for Inhomogeneously Broadened Raman Bands.

Ryan S Jakubek1

  • 1NASA Johnson Space Center, 538008Jacobs Technology Inc, Houston, TX, USA.

Applied Spectroscopy
|September 6, 2022
PubMed
Summary

Researchers developed a new method to accurately analyze inhomogeneously broadened Raman bands by modeling them as a continuum of homogeneous bands. This accounts for instrumental artifacts, improving data correlation and cross-instrument comparisons.

Keywords:
IRBRaman spectroscopyinhomogeneousintrinsic Raman bandmodelingslit function

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
07:44

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

15.2K

Related Experiment Videos

Last Updated: Aug 29, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.2K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
07:44

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

15.2K

Area of Science:

  • Spectroscopy
  • Chemical Physics
  • Materials Science

Background:

  • Raman spectroscopy provides detailed sample physics and chemistry via band parameters.
  • Instrumental artifacts complicate analysis and hinder cross-instrument comparisons.
  • Current methods for correcting instrumental effects are insufficient for inhomogeneously broadened bands.

Purpose of the Study:

  • To develop a method for accounting for instrumental effects on inhomogeneously broadened Raman bands.
  • To enable accurate correlation of Raman data to sample properties.
  • To facilitate reliable comparisons of Raman spectra across different instruments.

Main Methods:

  • Modeling inhomogeneous Raman bands as a continuum of homogeneous Raman bands.
  • Incorporating the effects of stochastic fluctuation energy wells.
  • Analyzing the influence of slit function, intrinsic Raman bands, and slit width on band parameters.

Main Results:

  • A novel model quantifies the impact of experimental parameters on inhomogeneously broadened Raman bands.
  • The method allows for the separation of instrumental effects from intrinsic sample properties.
  • Provides a framework for accurate analysis and comparison of complex Raman spectra.

Conclusions:

  • The derived model offers the first quantitative description of factors influencing inhomogeneously broadened Raman bands.
  • This advancement addresses a critical gap in Raman spectroscopy data analysis.
  • Enables more reliable scientific insights from Raman spectroscopy, particularly for complex materials.