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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

518
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
518
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

521
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
521

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Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
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Raman Spectroscopic Tools to Probe the Skin-(Trans)dermal Formulation Interface.

Hazel Garvie-Cook1, Magdalena Hoppel1, Richard H Guy1

  • 1Department of Life Sciences, University of Bath, Claverton Down, Bath BA2 7AY, U.K.

Molecular Pharmaceutics
|September 6, 2022
PubMed
Summary

Confocal Raman microspectroscopy analyzes topical and transdermal drug delivery. This technique visualizes drug penetration and transformation within the skin, aiding formulation optimization.

Keywords:
Raman imagingRaman spectroscopyskin bioavailabilitytopical skin formulationstransdermal drug delivery

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Area of Science:

  • Pharmaceutical Sciences
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Topical and transdermal drug products are complex formulations requiring precise methods to assess drug delivery.
  • Quantifying drug levels and kinetics within the skin is crucial for product development and efficacy.
  • Minimally invasive techniques are sought to evaluate drug absorption and clearance into systemic circulation.

Purpose of the Study:

  • To illustrate the application of confocal Raman microspectroscopy and imaging for analyzing topical and transdermal drug delivery.
  • To elucidate chemical properties influencing drug transfer at the formulation-skin interface.
  • To investigate drug product transformation, solubility changes, and skin absorption kinetics.

Main Methods:

  • Confocal Raman microspectroscopy and imaging were employed to study drug delivery systems.
  • Techniques included visualization, depth slicing, and profiling to analyze drug-skin interactions.
  • The study focused on the chemical properties of the delivery system and skin, drug transformation, and absorption.

Main Results:

  • Confocal Raman microspectroscopy provided insights into the chemical interactions at the formulation-skin interface.
  • The method allowed visualization of drug product transformation and changes in active compound solubility post-application.
  • Drug absorption into the skin and subsequent clearance into deeper layers were investigated.

Conclusions:

  • Confocal Raman microspectroscopy offers valuable qualitative and semi-quantitative data on topical and transdermal drug product performance.
  • The technique aids in understanding drug transfer, transformation, and skin penetration.
  • This approach supports the improvement and optimization of topical and transdermal drug formulations.