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Updated: Aug 29, 2025
A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage
Published on: July 28, 2018
The Effects of Necrostatin-1 on Cerebral Vasospasm- Induced Subarachnoid Hemorrhage
Mehmet Hakan Sahin1, Emin Akyuz, Hakan Hadi Kadioglu
1Ataturk University, School of Medicine, Department of Neurosurgery, Erzurum, Turkey.
Aim:
To investigate the effects of necrostatin-1 (NEC-1) on subarachnoid hemorrhage (SAH)-induced vasospasm in a rat model.
Material And Methods:
A total of 59 male Wistar albino rats were randomly divided into six groups: control (group 1), sham (group 2), decapitation one hour after SAH (group 3), decapitation 48 hours after SAH (group 4), NEC-1 given 15 minutes before SAH and decapitation one hour after SAH (group 5), and NEC-1 given 24 hours after SAH and decapitation 48 hours after SAH (group 6). NEC-1 (1 uq) was administered intracisternally in dimethyl sulfoxide (DMSO) (2.6 uq). After decapitation, the cross-sectional areas and wall thicknesses of basilar arteries were determined histopathologically using stereological methods.
Results:
NEC-1 administered before SAH had a statistically significant preventive effect on vasospasm following SAH. Arterial wall thicknesses were found to be significantly increased in the SAH without NEC-1 groups but not in the control group, the sham group or the NEC-1 groups.
Conclusion:
The results of this study show that NEC-1 can prevent vasospasm in rats and has cytoprotective effects. Further studies are needed for the clinical use of this agent.
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