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Updated: Aug 29, 2025
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An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
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Characterization and Genome Sequence of Arthrobacter Phage Iter
Kathleen A Daly1, Audrey E Nesbit1, Jacob D Buchholz1
1Department of Biology, University of North Georgia, Dahlonega, Georgia, USA.
Microbiology Resource Announcements
|September 6, 2022
Summary
Arthrobacter phage Iter, a novel virus, was discovered in North Georgia. This phage belongs to the AZ cluster of actinobacteriophages, showing high genetic similarity to Arthrobacter phage Phives.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Actinobacteriophages are viruses that infect bacteria belonging to the Actinobacteria phylum.
- Phage classification into clusters is based on nucleotide sequence identity, aiding in understanding viral diversity and evolution.
- The discovery of new phages contributes to the growing knowledge of bacteriophage populations and their genetic makeup.
Purpose of the Study:
- To report the isolation and initial characterization of a novel Arthrobacter phage, named Iter.
- To determine the genomic features of Arthrobacter phage Iter, including genome size, open reading frames (ORFs), and GC content.
- To classify Arthrobacter phage Iter within the broader context of actinobacteriophages based on genomic relatedness.
Main Methods:
- Isolation of Arthrobacter phage Iter from a soil sample in North Georgia.
- Whole-genome sequencing to determine the genome size, ORF count, and GC content.
- Comparative genomic analysis using nucleotide sequence identity to compare Iter with known actinobacteriophages.
Main Results:
- Arthrobacter phage Iter has a genome size of 43,963 base pairs (bp) with 70 open reading frames (ORFs).
- The GC content of the Iter genome is 67.4%.
- Iter shares 89.11% nucleotide identity with Arthrobacter phage Phives, placing it in cluster AZ of actinobacteriophages.
Conclusions:
- Arthrobacter phage Iter represents a newly identified member of the actinobacteriophage community.
- Its high genomic similarity to Phives confirms its placement within cluster AZ.
- This finding expands the characterized diversity of bacteriophages infecting Arthrobacter species.
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