A Basic and Simple Chromosome Preparation Protocol

Chromosome preparation for chromosome analysis is basic and indispensable for wide area of biology such as genetics, medicine, molecular biology, or other many fields. However, it seems that the many artisans in that fields feel hard to get start chromosome preparation technique, as it is something technically demanding or troublesome, or it needs something secret manner to prepare good quality of chromosome spreads. Actually many technical variations among individual laboratories exist. The aims of this chapter is to describe concise and minimal fundamental protocols specifically focused on human chromosome preparation, as step-by-step guiding with instructing essential points.