Related Experiment Video
Updated: Aug 29, 2025
Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
An intrinsic dual-emitting fluorescence sensing toward tetracycline with self-calibration model based on luminescent
Baoqian Shi1, Xinai Zhang1, Wenting Li1
1Agricultural Product Processing and Storage Lab, School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China; International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, Jiangsu University, Zhenjiang 212013, China.
Abstract:
A novel ratiometric fluorescence sensor based on lanthanide-functionalized metal-organic frameworks (Ag+/Eu3+@UiO-66(COOH)2, AEUC) with intrinsic dual-emitting bands was fabricated to determine tetracycline (TC) residues by exploiting (UiO-66-(COOH)2, UC) as reference units, Eu3+as recognition units and Ag+ as fluorescence enhancer. Benefiting from specific binding sites and functional adsorbent channels of AEUC, efficient capture and specific recognize toward TC could be achieved, remarkably enhancing the reliability. The synergistic effect of inner filter effect (IFE) and photoinduced electron transfer (PET) process between TC and AEUC was verified, enabling the detection limit and quantification limit of established strategy reaching as 12.8 nmol/L and 38.6 nmol/L and conspicuous fluorescence color gradation from red to blue. Given its portability, point-of-care sensing platform and nanoprobe-immobilized test-paper-based measurement allowing for on-site qualitative identification and semi-quantitative assay of TC was devised for sensing visualization and has been successfully applied in foodstuff samples, providing prospect in the field of smart devices for visual monitoring of TC.
