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Serum GFAP - reference interval and preanalytical properties in Danish adults
Lea Tybirk1, Claus Vinter Bødker Hviid1,2,3, Cindy Soendersoe Knudsen1
1Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
Clinical Chemistry and Laboratory Medicine
|September 6, 2022
Summary
Establishing reference intervals for serum glial fibrillary acidic protein (GFAP) is crucial for its clinical use in neurological diseases. This study provides age-dependent ranges and confirms GFAP stability under various preanalytical conditions.
Area of Science:
- Neurology
- Biomarker Discovery
- Clinical Chemistry
Background:
- Glial fibrillary acidic protein (GFAP) shows potential as a biomarker for neurological disease diagnosis and prognosis.
- Establishing reliable reference intervals and understanding preanalytical factor effects are critical for the clinical application of serum GFAP.
Purpose of the Study:
- To establish age-dependent reference intervals for serum GFAP in adults.
- To evaluate the impact of preanalytical factors on serum GFAP measurements.
Main Methods:
- Serum samples from 371 healthy adults (21-90 years) were analyzed using a single-molecule array (Simoa) assay.
- Continuous and traditional age-partitioned reference intervals were modeled and compared.
- Preanalytical stability was assessed under conditions including temperature, storage duration, freeze-thaw cycles, and hemolysis.
Main Results:
- Both continuous and age-partitioned reference intervals indicated increasing serum GFAP levels and variability with age.
- Established reference intervals were 25-136 ng/L (20-39 yrs), 34-242 ng/L (40-64 yrs), and 5-438 ng/L (65-90 yrs).
- Serum GFAP demonstrated good stability across tested preanalytical conditions, with minor variations observed.
Conclusions:
- Age-dependent reference ranges for serum GFAP in adults have been successfully established.
- Serum GFAP exhibits good stability, supporting its reliability as a biomarker in clinical settings.
