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Related Experiment Video

Updated: Aug 29, 2025

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
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Single Photon Kilohertz Frame Rate Imaging of Neural Activity.

Tian Tian1, Yifang Yuan1, Srinjoy Mitra2

  • 1Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, EH8 9XD, UK.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 6, 2022
PubMed
Summary

Single photon avalanche diode (SPAD) sensors enable high-speed imaging of neural activity. This technology captures millisecond-scale neural dynamics, advancing our understanding of brain function and disorders.

Keywords:
genetically encoded voltage indicators (GEVIs)kilohertz frame rateneural activityshot noisesingle photon avalanche diodes (SPADs)temporal binningvoltage imaging

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Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Engineering

Background:

  • Understanding cognition and neurological disorders necessitates precise spatiotemporal measurement of neural activity.
  • Genetically encoded voltage indicators (GEVIs) allow monitoring of neuronal spiking and subthreshold activity.
  • Standard camera systems struggle to achieve the millisecond-scale imaging required for GEVIs.

Purpose of the Study:

  • To introduce and evaluate single photon avalanche diode (SPAD) sensors for high-frame-rate neural imaging.
  • To demonstrate the capability of SPAD sensors in resolving fast neural dynamics using GEVIs.

Main Methods:

  • Utilized an array of SPAD sensors to image neurons expressing the GEVI Voltron-JF525-HTL.
  • Achieved imaging frame rates up to 10 kHz.
  • Applied SPAD imaging to an ex vivo seizure model.

Main Results:

  • SPAD sensors resolved subthreshold and spiking neural activity with shot-noise-limited signals at kilohertz frame rates.
  • Demonstrated millisecond-scale synchronization of neural activity in an ex vivo seizure model.
  • Confirmed the suitability of SPADs for capturing rapid neural events.

Conclusions:

  • SPAD sensors offer a powerful tool for high-speed neural imaging, overcoming limitations of conventional cameras.
  • This technology enables the investigation of millisecond timescale neural dynamics, crucial for understanding brain function.
  • SPAD imaging holds significant potential for neuroscience research and the study of neurological disorders.