Related Experiment Video

Updated: Aug 29, 2025

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.2K

Design of Personalized Neoantigen RNA Vaccines Against Cancer Based on Next-Generation Sequencing Data

Begoña Alburquerque-González1, María Dolores López-Abellán2, Ginés Luengo-Gil2

  • 1Pathology and Histology Department Facultad de Ciencias de la Salud, UCAM Universidad Católica San Antonio de Murcia, Murcia, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|September 6, 2022
PubMed

Insights

Therapeutic cancer vaccines using tumor neoantigens (TNAs) show promise. Advances in RNA vaccine technology enable rapid, personalized TNA vaccine development for tailored cancer treatment.

Area of Science:

  • Oncology
  • Immunology
  • Vaccinology

Background:

  • Immune checkpoint inhibitors (ICIs) show clinical success in cancer therapy.
  • The efficacy of ICIs is linked to tumor neoantigen (TNA) expression, which is often limited.
  • RNA vaccine technology has advanced, improving mRNA stability, immunogenicity, and delivery.

Purpose of the Study:

  • To explore the resurgence of therapeutic cancer vaccines.
  • To detail the pipeline for synthesizing TNA-carrying RNA vaccines for personalized cancer treatment.

Main Methods:

  • Next-generation sequencing (NGS) for individual tumor analysis.
  • Selection of immunogenic TNAs.
  • Nucleic acid synthesis and drug delivery system development.
  • Immunogenicity assessment of TNA-carrying RNA vaccines.

Main Results:

  • The synthesis pipeline integrates multiple steps for TNA vaccine creation.
  • Various alternatives and variations exist within each step of the pipeline.

Conclusions:

  • Combining ICI insights with RNA vaccine advancements enables personalized TNA cancer vaccines.
  • The described pipeline facilitates rapid development of tailored cancer vaccines.
Keywords:
AlgorithmCancerDrug delivery systemsHigh-throughput nucleotide sequencingImmune responseImmunotherapyNeoantigenVaccinesmRNA

More Related Videos

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles09:28

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

11.7K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

Related Concept Videos

Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
494
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.3K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.1K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.3K