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Updated: Aug 29, 2025

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
Constituents of Tinospora sinensis and their nitric oxide inhibitory activities
Bui Thi Mai Anh1, Do Thi Trang2, Hoang Thi Tuyet Lan1
1Department of Basic Science, University of Transport and Communications, Hanoi 11512, Vietnam.
Abstract:
One new phenylpropanoid glycoside, tinosinen A (1) and 13 known compounds, tinosinen (2), citrusin B (3), picraquassioside C (4), erythro-guaiacylglycerol-β-O-4'-coniferyl alcohol (5), erythro-guaiacylglycerol-8-O-4'-(sinapyl alcohol) ether (6), erythro-syringylglycerol-8-O-4'-(sinapyl alcohol) ether (7), seco-isolariciresinol 9-O-D-β-glucopyranoside (8), tinosposide A (9), pinoresinol-4'-O-β-D-glucopyranoside (10), syringaresinol-4'-O-β-D-glucopyranoside (11), pinoresinol (12), syringaresinol (13), and lirioresino-β-dimethyl ether (14) were isolated from the stems of Tinospora sinensis (Lour.) Merr. Their structures were established by detailed spectroscopic studies and comparisons with those reported in the literature. Compound 13 showed significant inhibitory NO production (IC50 value of 38.53 ± 1.90 μM) in RAW264.7 macrophages, LPS-stimulated. Compounds 3-7, 11, 12, and 14 inhibited NO production with IC50 values ranging from 38.53 to 99.07 μM.
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