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Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
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A spatiotemporal transcriptomic network dynamically modulates stalk development in maize.

Liang Le1,2, Weijun Guo1, Danyao Du1

  • 1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Plant Biotechnology Journal
|September 7, 2022
PubMed
Summary

Researchers mapped maize stalk development by analyzing gene expression across all stalk internodes. This revealed key genes controlling stalk length and thickness, offering valuable insights for improving maize crops.

Keywords:
ZmD1maizeplant heightstalktranscriptome

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Area of Science:

  • Plant biology
  • Genomics
  • Agricultural science

Background:

  • Maize stalk development is crucial for lodging resistance and high-density planting.
  • Understanding the spatiotemporal regulation of maize stalk growth is essential for crop improvement.

Purpose of the Study:

  • To elucidate the spatiotemporal dynamics and regulatory networks governing maize stalk development.
  • To identify candidate genes influencing stalk length and thickness.

Main Methods:

  • Spatiotemporally resolved transcriptomics of developing maize stalks.
  • Weighted gene coexpression network analysis (WGCNA) to construct regulatory networks.
  • CRISPR/Cas9 gene editing for functional validation.

Main Results:

  • Identified four distinct developmental zones and nine gene expression clusters within maize internodes.
  • Constructed fine-resolution transcriptional regulatory networks.
  • Validated the role of a cytochrome P450 gene (ZmD1) in regulating stalk dimensions.

Conclusions:

  • Maize stalk development involves complex genetic regulation.
  • Identified key genes and modules for stalk length and thickness, providing resources for maize genetic improvement.