Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

103.0K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.0K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

73
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
73
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D chromatin compartment of round spermatids encodes the spatiotemporal program of histone-to-protamine exchange in spermiogenesis.

bioRxiv : the preprint server for biology·2026
Same author

A Rare T-Cell Factor 4 Lineage-negative Epithelial Stem Cell Supports Wound Repair and APC-deletion-induced Colon Tumorigenesis.

bioRxiv : the preprint server for biology·2026
Same author

Asymmetric histone inheritance regulates olfactory stem cell fates during regeneration.

Nature communications·2026
Same author

ZCWPW1 organizes telomeric architecture to drive meiotic chromosome movements.

bioRxiv : the preprint server for biology·2026
Same author

A hidden protamine PTM code in sperm generates heterogeneous chromatin states and finetunes reproductive fitness.

bioRxiv : the preprint server for biology·2025
Same author

Integrative multi-omics uncovers skeletal muscle enhancer programming of cardiorespiratory fitness.

bioRxiv : the preprint server for biology·2025
Same journal

Properties and Prospects of B Chromosomes.

Annual review of genetics·2026
Same journal

Lessons From Yeast: Mechanisms of Telomere Length Regulation.

Annual review of genetics·2026
Same journal

Mechanisms and Evolutionary Advantages of Unlimited Reproductive Lifespans in Naked Mole-Rat Queens.

Annual review of genetics·2026
Same journal

Impact of Small RNA Sponges on Regulatory RNA Networks in Bacteria.

Annual review of genetics·2025
Same journal

Context Specificity of MAP3K DLK Signaling in the Nervous System: Insights from Genetics and Genomics.

Annual review of genetics·2025
Same journal

The Field of Hair Cell Regeneration Is Ready for Input from Genomics and Epigenetics.

Annual review of genetics·2025
See all related articles

Related Experiment Video

Updated: Aug 29, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K

Decoding the Spermatogenesis Program: New Insights from Transcriptomic Analyses.

Mashiat Rabbani1, Xianing Zheng1, Gabe L Manske2

  • 1Department of Human Genetics, University of Michigan, Ann Arbor, Michigan, USA;

Annual Review of Genetics
|September 7, 2022
PubMed
Summary
This summary is machine-generated.

Single-cell RNA sequencing reveals testis cell diversity and developmental states, aiding interspecies comparisons for understanding spermatogenesis and infertility. Spatial analysis further illuminates germ cell-somatic cell interactions for therapeutic development.

Keywords:
evolutioninfertilitymeiosisscRNA-seqspermatogenesistranscription

More Related Videos

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K
Author Spotlight: Whole-Mount Fluorescence In Situ Hybridization to Study Spermatogenesis in the Anopheles Mosquito
05:31

Author Spotlight: Whole-Mount Fluorescence In Situ Hybridization to Study Spermatogenesis in the Anopheles Mosquito

Published on: May 26, 2023

2.5K

Related Experiment Videos

Last Updated: Aug 29, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K
Author Spotlight: Whole-Mount Fluorescence In Situ Hybridization to Study Spermatogenesis in the Anopheles Mosquito
05:31

Author Spotlight: Whole-Mount Fluorescence In Situ Hybridization to Study Spermatogenesis in the Anopheles Mosquito

Published on: May 26, 2023

2.5K

Area of Science:

  • Reproductive Biology
  • Genomics
  • Cellular Biology

Background:

  • Spermatogenesis is a complex process challenging to study due to cellular heterogeneity, limiting molecular profiling.
  • Bulk transcriptomic analyses struggle to capture stage-specific germ and somatic cell information.
  • Understanding spermatogenesis regulation and interspecies conservation is hindered by data limitations.

Purpose of the Study:

  • To leverage single-cell RNA sequencing (scRNA-seq) for detailed molecular profiling of testicular cells.
  • To create fine-grained cell atlases for understanding germ cell development and interspecies comparisons.
  • To explore germ cell-somatic cell interactions using advanced spatial analysis technologies.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) to analyze cell type diversity and molecular signatures.
  • Development of high-resolution testis cell atlases across species.
  • Application of multiplexed spatial analysis for tissue-level interaction studies.

Main Results:

  • scRNA-seq identified diverse known and novel cell types within the testis.
  • Established cell atlases define functional states along the germ cell developmental trajectory.
  • Spatial analysis technologies are beginning to resolve germ cell-somatic cell interactions.

Conclusions:

  • Single-cell studies significantly improve the understanding of gametogenesis.
  • Testicular cell atlases facilitate interspecies comparisons to uncover conserved mechanisms.
  • These advancements are crucial for investigating infertility causes and developing novel therapeutic strategies.