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Discriminating normal and inflammatory mice models by viscosity changes with a two-photon fluorescent probe.

Xinya Hao¹, Jingting Zhan¹, Chen Geng¹

¹Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|September 7, 2022

Summary

Researchers developed a new near-infrared fluorescent probe (HCT) for sensitive intracellular viscosity imaging. This probe successfully distinguished between normal and inflamed tissues in living organisms, showing promise for biomedical applications.

Keywords:

Fluorescent probe Inflammation Two-photon Viscosity

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Area of Science:

Biomedical Engineering
Molecular Imaging
Fluorescence Spectroscopy

Background:

Intracellular viscosity changes are linked to various diseases.
Developing tools for intracellular viscosity measurement is crucial for clinical diagnostics.
Existing methods may lack sensitivity or applicability in living systems.

Purpose of the Study:

To design and synthesize a novel two-photon near-infrared fluorescent probe (HCT) for intracellular viscosity imaging.
To evaluate the probe's sensitivity, photostability, and cytotoxicity.
To demonstrate its utility in distinguishing normal from inflamed biological models.

Main Methods:

Synthesis of the HCT fluorescent probe.
Characterization of probe's photophysical properties.
In vitro experiments using living cells for fluorescence imaging.
In vivo experiments using living tissues and animal models (mice).

Main Results:

The HCT probe exhibited high sensitivity for viscosity detection.
HCT demonstrated excellent photostability and low cytotoxicity in cellular imaging.
Successful single-photon and two-photon fluorescence imaging of intracellular viscosity in living cells was achieved.
The probe effectively visualized viscosity differences in living tissues and discriminated between normal and inflamed mice.

Conclusions:

The developed HCT probe is a sensitive and reliable tool for intracellular viscosity imaging.
Its favorable properties enable applications in both cellular and in vivo settings.
HCT holds significant potential for biomedical applications, particularly in diagnosing inflammatory conditions.