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Updated: Aug 29, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

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m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation

Sung Ho Boo1, Hongseok Ha1, Yoon Ki Kim2

  • 1Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.

Cell Reports
|September 7, 2022
PubMed

Insights

N1-methyladenosine (m1A) RNA modification accelerates the degradation of N6-methyladenosine (m6A) modified mRNAs. HRSP12 protein binding to m1A enhances m6A reader YTHDF2 interaction, promoting mRNA decay.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Epigenetics

Background:

  • N6-Methyladenosine (m6A) is the most prevalent internal mRNA modification, regulating gene expression.
  • YTHDF2 protein binds m6A on mRNAs, leading to rapid mRNA degradation via recruited RNA-degrading enzymes.

Purpose of the Study:

  • To investigate the role of N1-methyladenosine (m1A) in RNA modification pathways.
  • To elucidate the mechanism by which m1A influences m6A-mediated RNA degradation.

Main Methods:

  • Identification of RNA-binding proteins interacting with m1A.
  • Analysis of protein-RNA interactions using transcriptome-wide approaches.
  • Assessment of mRNA and circular RNA degradation dynamics.

Main Results:

  • N1-methyladenosine (m1A) accelerates m6A-mediated rapid mRNA degradation.
  • HRSP12 protein was identified as an m1A reader, promoting YTHDF2 binding to m6A.
  • HRSP12-dependent downregulation of mRNAs and circular RNAs containing both m1A and m6A was observed.

Conclusions:

  • m1A acts as a crucial regulator of m6A RNA degradation.
  • Crosstalk between different RNA modifications (m1A and m6A) influences gene expression.
  • HRSP12 mediates the interplay between m1A and m6A modifications to control RNA stability.
Keywords:
CP: Molecular biologyHRSP12N(1)-methyladenosineN(6)-methyladenosineRNase P/MRPYTHDF2m(1)Am(6)AmRNA decay

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