Related Experiment Video
Updated: Aug 29, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Anoxic-Aerobic-Anoxic sequencing batch reactor for enhanced nitrogen removal
Susan N James1, Arya Vijayanandan1
1Department of Civil Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
This study optimized an anoxic-aerobic-anoxic process for simultaneous removal of organic carbon and nitrogen in wastewater. The process achieved high removal efficiencies, demonstrating cost-effective wastewater treatment.
Area of Science:
- Environmental Science
- Environmental Engineering
- Microbiology
Background:
- Wastewater treatment requires efficient removal of organic carbon and nitrogen.
- Simultaneous removal is challenging and often energy-intensive.
Purpose of the Study:
- To establish and optimize an anoxic-aerobic-anoxic process for simultaneous organic carbon and nitrogen removal.
- To understand the biochemical reactions and microbial communities involved.
Main Methods:
- Utilized a sequencing batch reactor with an anoxic-aerobic-anoxic configuration.
- Optimized dissolved oxygen (DO) levels and anoxic periods.
- Conducted inhibition studies and microbial community analysis.
Main Results:
- Achieved TOC, NH4+-N, and TN removal efficiencies of 98.76%, 98.52%, and 88.23% respectively.
- Identified optimal DO of 1.5 mg/L and 1-hour pre- and post-anoxic periods.
- Confirmed heterotrophic nitrification/direct conversion and aerobic denitrification as key pathways.
Conclusions:
- The anoxic-aerobic-anoxic process effectively removes organic carbon and nitrogen simultaneously.
- Limited aeration and varied redox conditions are beneficial for nitrogen removal, reducing energy costs.
- Microbial community analysis supports the identified nitrogen removal mechanisms.
More Related Videos
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
11:31Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Related Concept Videos
Metabolism of Chemolithotrophs
Inorganic Nitrogen Assimilation
Bioremediation