A proteomic and RNA-seq transcriptomic dataset of capsaicin-aggravated mouse chronic colitis model

Area of Science:

• Gastroenterology and Immunology
• Molecular Biology

Background:

• Dietary factors significantly influence inflammatory bowel disease (IBD) risk and progression.
• Capsaicin, a component of spicy foods, is linked to IBD symptom exacerbation and neutrophil activation in the gut.

Purpose of the Study:

• To investigate the molecular mechanisms underlying capsaicin-induced IBD relapse.
• To identify signaling pathways and cellular responses involved in capsaicin's effect on intestinal inflammation and neutrophil activation.

Main Methods:

• Proteomic analysis of intestinal tissues from mice with chronic colitis after capsaicin administration.
• Transcriptomic analysis of dHL-60 cells stimulated with capsaicin.

Main Results:

• Multiomic analyses revealed specific proteins and genes associated with capsaicin-induced disease flares.
• Identified potential molecular targets involved in capsaicin's pro-inflammatory effects on intestinal tissues and immune cells.

Conclusions:

• Capsaicin plays a role in IBD pathogenesis through specific molecular pathways.
• Findings provide novel insights into the mechanisms of dietary triggers in IBD, suggesting avenues for therapeutic research.