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Published on: June 13, 2020
The potential therapeutic effect of klotho on cell viability in human colorectal adenocarcinoma HT-29 cells
Ayla Eker Sariboyaci1,2,3, Onur Uysal1,2,3, Merve Nur Soykan1,2
1Cellular Therapy and Stem Cell Production Application, Research Centre (ESTEM), Institute of Health Sciences, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey.
Abstract:
Klotho is an anti-aging, anti-inflammator, and anti-oxidative protein and has been shown to important role in tumorigenesis, proliferation, survival, autophagy, and resistance to tumor suppressor effects in several types of human cancers. In this study, we aimed to investigate possible anti-tümör and apoptotic effects of exogen klotho in human colorectal adenocarcinoma cells (HT-29) and healthy colon cells (CCD 841 CoN). The WST-8 test was used to determine the half-maximum inhibitory concentration (IC50) of the klotho protein. AO-PI fluorescent staining techniques and Annexin V-PI flow cytometry was utilized to observe and detect the apoptosis of cancer cells induced by klotho. Our results demonstrated that klotho had a cytotoxic effect against colorectal adenocarcinoma cells in a dose-dependent manner. Our Annexin V-PI flow cytometric and AO-PI fluorescent analyses showed that klotho induced quantitative and morphological changes that indicate apoptotic induction in the human colorectal adenocarcinoma. This study results proved for the first time that klotho may be an effective potential therapeutic agent that may be used in adjuvant therapy in human colorectal adenocarcinoma it does not affect selectively healthy colon cells and but leading cancer cells to apoptosis.
Insights
Klotho protein exhibits anti-cancer properties by inducing apoptosis in human colorectal adenocarcinoma cells. This study suggests klotho as a potential therapeutic agent for colorectal cancer without harming healthy colon cells.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Klotho protein possesses anti-aging, anti-inflammatory, and anti-oxidative properties.
- Klotho plays a significant role in tumorigenesis, cell proliferation, survival, autophagy, and resistance to tumor suppressor mechanisms in various human cancers.
Purpose of the Study:
- To investigate the potential anti-tumor and apoptotic effects of exogenous klotho in human colorectal adenocarcinoma cells (HT-29) and healthy colon cells (CCD 841 CoN).
Main Methods:
- Determined the half-maximum inhibitory concentration (IC50) of klotho using the WST-8 assay.
- Utilized AO-PI fluorescent staining and Annexin V-PI flow cytometry to assess apoptosis in cancer cells induced by klotho.
Main Results:
- Klotho demonstrated a dose-dependent cytotoxic effect on colorectal adenocarcinoma cells.
- Annexin V-PI flow cytometry and AO-PI fluorescent staining revealed that klotho induced quantitative and morphological changes indicative of apoptosis in human colorectal adenocarcinoma cells.
- Klotho selectively targeted cancer cells, leading to apoptosis without affecting healthy colon cells.
Conclusions:
- Klotho exhibits potential as an effective therapeutic agent for adjuvant therapy in human colorectal adenocarcinoma.
- Klotho selectively induces apoptosis in colorectal cancer cells, offering a targeted therapeutic approach.
