Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional Bursting in Pluripotent Stem Cells.

Biology·2026
Same author

Carnosic Acid Activates the STING/IRF3 Pathway to Induce Nitric Oxide-Mediated Apoptosis in Osteosarcoma Cells.

Antioxidants (Basel, Switzerland)·2026
Same author

Preparation of <i>Artemisia argyi</i>-Derived Extracellular Nanovesicles and Their Protective Effects Against Oxidative Stress-Induced Senescence in Endometrial Stromal Cells.

Bioengineering (Basel, Switzerland)·2026
Same author

Chitosan-Functionalized Magnetic Graphene Oxide Nanocomposites Enable High-Purity mRNA Enrichment To Avoid False Positives in LC-MS/MS Modification Profiling.

Analytical chemistry·2026
Same author

FOXM1 Maintains Homeostasis and Self-Renewal in Wharton's Jelly Mesenchymal Stem Cells.

Genes·2025
Same author

Propiolates-Based Selective Labeling and Affinity Capture Enables High-Fidelity Transcriptome-Wide Profiling of A-to-I RNA Editing.

Bioconjugate chemistry·2025

Related Experiment Video

Updated: Aug 29, 2025

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
10:00

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Published on: December 11, 2020

2.6K

Epigenetic Alterations under Oxidative Stress in Stem Cells.

Min Huang1, Qiang Wu1, Zhi-Hong Jiang1

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, SAR, China.

Oxidative Medicine and Cellular Longevity
|September 8, 2022
PubMed
Summary

Oxidative stress impacts stem cells by altering DNA methylation and histone modifications. Epigenetic changes are crucial for stem cell behavior under oxidative stress, suggesting a reciprocal influence.

More Related Videos

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.1K
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.2K

Related Experiment Videos

Last Updated: Aug 29, 2025

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
10:00

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

Published on: December 11, 2020

2.6K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.1K
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.2K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Stem Cell Research

Background:

  • Oxidative stress, an imbalance between reactive oxygen species (ROS) and antioxidant defenses, is implicated in human diseases.
  • Stem cells possess self-renewal and differentiation capabilities, making them sensitive to environmental changes.
  • Epigenetic mechanisms, including DNA methylation and histone modifications, regulate gene expression and cellular responses.

Purpose of the Study:

  • To review recent findings on the effects of oxidative stress on epigenetic modifications in stem cells.
  • To explore the interplay between oxidative stress and epigenetic alterations in stem cell behavior.

Main Methods:

  • Literature review of studies investigating oxidative stress and epigenetics in stem cells.
  • Analysis of research on DNA methylation and histone modifications under oxidative conditions.

Main Results:

  • Oxidative stress induces changes in DNA methylation patterns in stem cells.
  • Histone modifications are significantly altered in stem cells exposed to oxidative stress.
  • These epigenetic alterations influence stem cell homeostasis and fate decisions.

Conclusions:

  • Epigenetic alterations are key mediators of stem cell responses to oxidative stress.
  • A reciprocal relationship likely exists between oxidative stress and epigenetic modifications in stem cells.