Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eosinophils do respond to fMLP.

M Yazdanbakhsh, C M Eckmann, L Koenderman

    Blood
    |August 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    No difference in glycosphingolipid metabolism and mitochondrial function in glucocorticoid-induced insulin resistance in healthy men.

    The Journal of clinical endocrinology and metabolism·2013
    Same author

    Current practise of testing for inherited thrombophilia.

    Journal of thrombosis and haemostasis : JTH·2007
    Same author

    Bilirubin oxidase as a solution for the interference of hyperbilirubinemia with ADAMTS-13 activity measurement by FRETS-VWF73 assay.

    Journal of thrombosis and haemostasis : JTH·2007
    Same author

    Mitochondria in neutrophil apoptosis.

    International journal of hematology·2006
    Same author

    Prolonged storage of red blood cells affects aminophospholipid translocase activity.

    Vox sanguinis·2006
    Same author

    Differential sensitivities of pathogens in red cell concentrates to Tri-P(4)-photoinactivation.

    Vox sanguinis·2006
    Same journal

    Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

    Blood·2026
    Same journal

    Identifying steroid-refractory aGVHD before it happens.

    Blood·2026
    Same journal

    ELISA-negative HIT: antibody recognition and relevance.

    Blood·2026
    Same journal

    EBV and immunodeficiency: the odd couple drawn to the brain.

    Blood·2026
    Same journal

    A bone to pick with ferric carboxymaltose.

    Blood·2026
    Same journal

    A step toward streamlining HIT diagnosis.

    Blood·2026
    See all related articles

    Normal human eosinophils respond to formyl-methionyl-leucyl-phenylalanine (fMLP), demonstrating increased intracellular calcium and oxygen consumption. However, fMLP should not be used for eosinophil isolation due to cellular deactivation.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Eosinophils are key immune cells involved in inflammatory responses.
    • The chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP) is known to activate neutrophils.
    • The response of eosinophils to fMLP requires further investigation.

    Purpose of the Study:

    • To investigate the functional response of purified normal human eosinophils to fMLP.
    • To determine the optimal conditions and efficacy of fMLP as a stimulus for eosinophils.
    • To assess the impact of fMLP exposure on eosinophil viability and subsequent responsiveness.

    Main Methods:

    • Isolation of highly pure eosinophil suspensions (>95%) from normal human blood using Percoll gradients.
    • Stimulation of eosinophils with varying concentrations of fMLP.

    Related Experiment Videos

  • Measurement of intracellular free calcium, oxygen consumption, nitroblue tetrazolium reduction, and chemiluminescence as indicators of cellular activation.
  • Comparison of eosinophil responses to those of neutrophils from the same blood samples.
  • Assessment of eosinophil responsiveness after pre-incubation with fMLP.

    • Main Results:

    • Normal human eosinophils exhibit a dose-dependent response to fMLP ( > 10(-7) mol/L), including increased intracellular calcium, oxygen consumption, and chemiluminescence.
    • Eosinophil maximal response to fMLP was lower than neutrophils and required higher fMLP concentrations (at least 10-fold).
    • Low fMLP concentrations (approx. 10(-8) mol/L) primed eosinophils and neutrophils for enhanced response to other stimuli.
    • Eosinophils treated with fMLP during purification lost their subsequent responsiveness to fMLP.

    Conclusions:

    • Normal human eosinophils are responsive to fMLP, albeit with lower efficacy compared to neutrophils.
    • fMLP can prime eosinophils and neutrophils, enhancing their response to secondary stimuli.
    • The deactivating effect of fMLP on eosinophils suggests it is unsuitable for their isolation.