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An inhibitory monoclonal antibody against human tissue factor
Blood
|August 1, 1987
Summary
A new monoclonal antibody, HTF1-7B8, inhibits tissue factor activity and can detect tissue factor in human and bovine samples. This antibody is valuable for purifying and characterizing tissue factor structure and function.
Area of Science:
- Biochemistry
- Immunology
- Molecular Biology
Background:
- Tissue factor initiates the coagulation cascade.
- Monoclonal antibodies are crucial tools for protein research and diagnostics.
Purpose of the Study:
- To develop and characterize a novel monoclonal antibody against human tissue factor.
- To investigate the antibody's utility in detecting and purifying tissue factor.
Main Methods:
- Hybridoma technology was used to generate the antibody HTF1-7B8.
- The antibody's inhibitory activity and epitope specificity were assessed.
- Affinity chromatography using the antibody was employed for tissue factor purification.
Main Results:
- The monoclonal IgG1 antibody HTF1-7B8 inhibits tissue factor procoagulant activity.
- HTF1-7B8 detects conserved epitopes on human and bovine tissue factor.
- Purified tissue factor from human brain and placenta showed identical biochemical properties.
Conclusions:
- The HTF1-7B8 antibody is a potent inhibitor and a reliable detection tool for tissue factor.
- This antibody facilitates immunopurification and structural characterization of tissue factor.
- HTF1-7B8 is a valuable reagent for studying tissue factor biology and the coagulation pathway.