Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fluorescent sensors for imaging of interstitial calcium.

Nature communications·2023

Same author

Early calcium and cardiac contraction defects in a model of phospholamban R9C mutation in zebrafish.

Journal of molecular and cellular cardiology·2022

Same author

Epigenetic promoter alterations in GI tumour immune-editing and resistance to immune checkpoint inhibition.

Gut·2021

Same author

Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.

Cell chemical biology·2020

Same author

High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators.

iScience·2019

Same author

Molecular characterization of an aggregation-prone variant of alpha-synuclein used to model synucleinopathies.

Biochimica et biophysica acta. Proteins and proteomics·2019

Same journal

Diving exposure and pulmonary stress.

The Journal of physiology·2026

Same journal

Systems modelling of mitochondrial dynamics in different exercise regimes.

The Journal of physiology·2026

Same journal

Central leptin resistance precedes obesity and drives early endocrine dysfunction.

The Journal of physiology·2026

Same journal

Decoding the molecular memory of obesity using machine learning and microRNA dynamics.

The Journal of physiology·2026

Same journal

Kinematic-calcium loops unravel impaired excitation-contraction coupling in MELAS-affected cardioids.

The Journal of physiology·2026

Same journal

hERG1 channels and potential therapeutics for long QT syndrome.

The Journal of physiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 29, 2025

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Probing the interstitial calcium compartment.

Ariel A Valiente-Gabioud¹, Arne Fabritius¹, Oliver Griesbeck¹

¹Tools for Bio-Imaging, Max-Planck-Institute for Biological Intelligence (i.F.), Martinsried, Germany.

The Journal of Physiology

|September 8, 2022

Summary

This summary is machine-generated.

Monitoring interstitial calcium is vital for understanding cellular processes. New genetically encoded calcium indicators offer advanced methods for studying calcium in challenging intercellular spaces.

Keywords:

bio-imaging calcium fluorescent proteins protein engineering

More Related Videos

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Published on: December 8, 2013

Related Experiment Videos

Last Updated: Aug 29, 2025

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Published on: May 19, 2017

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Published on: December 8, 2013

Area of Science:

Physiology
Cell Biology
Biochemistry

Background:

Interstitial fluid calcium is essential for cellular processes, acting as a key ion pool for cell entry and receptor sensing.
Global calcium homeostasis is understood, but local calcium handling in interstitial spaces requires further investigation.
Current methods for monitoring interstitial calcium are limited, necessitating new technological approaches.

Purpose of the Study:

To review existing methodologies for monitoring interstitial calcium.
To highlight novel approaches for studying local calcium handling.
To explore the potential of new technologies for in vivo calcium imaging.

Main Methods:

Review of current literature on interstitial calcium monitoring techniques.
Discussion of emerging technologies, specifically genetically encoded calcium indicators (GECIs).
Focus on low-affinity, high-performance GECIs for improved imaging capabilities.

Main Results:

Existing methods for interstitial calcium measurement present challenges in small, isolated spaces.
New generations of GECIs show promise for overcoming these limitations.
These advanced indicators could enable imaging of calcium in previously inaccessible intercellular structures.

Conclusions:

Further technological development is crucial for understanding local calcium dynamics.
Genetically encoded calcium indicators offer a powerful tool for real-time imaging of interstitial calcium.
This approach holds potential for advancing research in live tissues and organisms.