A retinal whole-mount method useful in detecting retrogradely-labeled ganglion cells
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Brain Research Bulletin
|April 1, 1987
Abstract:
The present report describes a simple retinal whole-mount method that is compatible with horseradish peroxidase histochemistry. The retina is removed unfixed, flattened and fixed. Histochemical procedures are carried out with the retina free-floating, prior to affixing it to the slide with formaldehyde vapor. The method consistently yields whole-mounts free of pigment epithelium and firmly affixed to the slide, with good preservation of retinal morphology and surface topography.